Gateway manual invitrogen

Invitrogen™ Gateway™ LR Clonase™ II enzyme mix is a proprietary enzyme and buffer formulation containing the bacteriophage lambda recombination proteins Integrase (Int) and Excisionase (Xis), the E. coli-encoded protein Integration Host Factor (IHF). Life Technologies, a Division of Invitrogen Corporation www.doorway.ru Choosing Products to Build GATEWAY™ Expression Clones as described in the GATEWAY Cloning Technology Instruction Manual, but does not provide rights to synthesize File Size: KB. A large selection of Gateway® destination vectors is available from Invitrogen to facilitate expression of your gene of interest in virtually any protein expression system. For more information about the vectors available, refer to the Gateway® Technology Central application portal on our Web site at www.doorway.ru or contactFile Size: KB.

TOPO TA Cloning—To create a Gateway entry clone. Step 1—Produce PCR product. Produce PCR products using Taq polymerase and your own protocol. End the PCR reaction with a final 7 to 30 minute extension step. Step 2—Perform the TOPO Cloning Reaction. Set up one of the following Invitrogen TOPO Cloning reactions using the reagents in the. Gateway Recombination Cloning Technology. Invitrogen Gateway recombination cloning uses a one hour reversible recombination reaction, without using restriction enzymes, ligase, subcloning steps, or screening of countless colonies, thereby saving you time, money, and effort. Benefits include: New advancements such as MultiSite Gateway Technology. Instruction Manual Gateway® pENTR™ Vectors Catalog nos. , , , , Version B 23 November A Limited Label License covers this product (see Purchaser.

Traditionally, gene cloning has relied on restriction enzyme digestion and ligation. In recent years, however, Gateway® cloning technology (Invitrogen Co.) has. Gateway® LR. Recombination. Reaction. IMPORTANT: This kit contains a new enzyme (LR Clonase II Plus) Please follow the protocol below and on pages 3 oct Software- Go to www.doorway.ru for detailed instructions to get started using Vector NTI Advance™ sequence analysis.